Scientist, Neurotoxin & Biologics Process Sciences

Company: AbbVie
Location: Irvine
Posted on: May 16, 2022

Job Description:

Scientist, Molecular Biology and Fermentation, Irvine, California Research & Development Job Description About AbbVie AbbVie (NYSE: ABBV) is a global, research-driven biopharmaceutical company committed to developing innovative advanced therapies for some of the world's most complex and critical conditions. The company's mission is to use its expertise, dedicated people and unique approach to innovation to markedly improve treatments across four primary therapeutic areas: immunology, oncology, virology and neuroscience. In more than 75 countries, AbbVie employees are working every day to advance health solutions for people around the world. For more information about AbbVie, please visit us at . Follow on Twitter, Facebook or LinkedIn . Envision working with energetic colleagues and inspirational leaders. Now, place yourself in that mix; leading discussions, asking the right questions and driving results. What Your New Manager Wants You To Know The Scientist, Molecular Biology and Fermentation will be an instrumental team member of the Neurotoxin & Biologics Process Sciences Department, based in Irvine, CA. The primary role of the scientist is to lead molecular biology activities including expression vector construction, cell line engineering and genetic verification of products for the use in bacterial biologics development. The scientist will need to design and execute studies for the evaluation of vector types/designs, promoters, strain optimization, gene expression, genome manipulation (deletions and knockout) in wild type bacteria and strategies for the evaluation of novel recombinant proteins with the intent to optimize product titer while minimizing process related impurities. Additional experience in microbial physiology and mapping metabolism pathways would be preferred. This role will include cell banking strategies for research and early development studies and will continue to support cell banks through tech transfer and used in clinical trials as a subject matter expert. Experience with genetic sequencing would be considered a plus. Additional responsibilities include data analysis, troubleshooting process and equipment, participation in project teams, lab inventory and organization, maintaining detailed records of batches and experiments. The secondary role of the scientist is to independently plan, design studies using Design of Experiment (DOE) strategies, execute experiments and develop processes to support the fermentation of natural and recombinant proteins from bacterial cell cultures. The scientist will support studies at small and intermediate scale during various stages of development including process characterization. The fermentation scientist will independently operate shake flask and small-scale upstream equipment (Ambr - 250, Biostat - B-DCU, single use fermenters, etc.). An ideal candidate would have the ability to work as an integral part of an upstream team and supporting drug discovery and downstream teams as well as demonstrate a high degree of flexibility, creativity, and initiative applied to the various duties and aspects of position. Furthermore, the position requires the individual to work with regulated Select Agents and Toxins access (i.e., Biosafety level 2 and biosafety level 3 laboratories). Access to and use of select agents is regulated by federal policies. Therefore, the candidate must conduct all work activities in compliance with AbbVie internal and applicable regulatory requirements including health screening and background checks. AbbVie internal requirements include compliance with ethics, environmental health and safety, financial, human resources, and general business policies, requirements, and objectives. YOU ARE more than just a title, YOU ARE--- A strategic thinker: align and excite internal stakeholders to facilitate success through strong leadership, strategic thinking and personal drive. First class team player: collaborate across multiple disciplines to ensure compliance and ability to influence cross-functional teams and senior leadership. You Will

• Have hands on laboratory bench work to develop and prepare biologics products candidates for various groups with tight timelines.
• Design and execute studies to support a novel product pipeline, including analysis and documentation, to support biologics drug development.
• Independently design expression vectors for the expression of proteins, as well as verify the construction and expression of produced vectors. Position requires proficient knowledge and hands on experience of plasmid construction, cell line transformation, expression optimization strategies and ability to map native expression pathways.
• Perform routine fermentation operations relating to fermenter prep: assembly/disassembly, P-test/SIP/CIP, in process sampling and media/buffer/reagent prep and able to troubleshoot operational and equipment issues.
• Willing to work with and handle potent neurotoxins and in-process samples that may contain toxin-expressing organisms.
• Ability to perform aseptic cell propagation and manipulation under a biosafety laminar flow hood.
• Follow study protocols and execute experiments independently (or minimal supervision) at small scale and large scales.
• Author and edit development reports and standard operating procedures. Compile experimental batch records and documents/inventory for in-process samples in the inventory system.
• Individual will evaluate lab equipment and procure the necessary supplies to ensure continual successful lab operations.
• Collaborate effectively to achieve results as an individual as well as part of a team environment. Assist fermentation team in tech transfer for cell lines and acceptance testing to support early-stage process development laboratory and initial clinical trials (internally or externally).
• Complete training consistent with safety, technical, operational, process, and other skill/ knowledge requirements for the position. Maintain training currency. Support safety program and ensure safety in all routine and new operations by strictly following SOPs.

Keywords: AbbVie, Irvine , Scientist, Neurotoxin & Biologics Process Sciences, Other , Irvine, California